Continuous Protein Crystallization in Mixed-Suspension Mixed-Product-Removal Crystallizers

The metastable needlelike form of lysozyme can be obtained from continuous crystallization in mixed-suspension mixed-product-removal (MSMPR) crystallizers for conditions at which the stable tetragonal is batch crystallization. A 3D-printed airlift crystallizer and stirred tank are used, both approximate an MSMPR well. influence residence time, precipitant concentration, agitation mechanism on attainable solid-state presented. crystals appear after nucleation form. An earlier transition to observed compared a conventional crystallizer, possibly caused by reduced attrition presence gas–liquid interface crystallizer. Furthermore, long time high concentration favor formation crystals. This work demonstrates how mode operation well-mixed affects model protein. results generally show importance obtaining quantitative understanding dynamic interplay between stochastic primary processes deterministic growth secondary so that future designed operated robustly produce protein desired